Coding

Part:BBa_K3621002

Designed by: Sangita Vasikaran   Group: iGEM20_MIT   (2020-10-04)


Cas6-> cleaves cut site

CRISPR-based endoribonuclease (ERN) developed for use in the PERSIST platform, cleaving at a cut site. Citation: 10.1101/2019.12.15.867150

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NotI site found at 71
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NgoMIV site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None